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Isolation and Genotyping of Acanthamoeba from Soil Samples in Markazi Province, Iran

Mehri Meighani1, Zahra Eslamirad2, Reza Hajihossein3

  • 1Department of Biology, School of Basic Sciences, Islamic Azad University, Arak Branch, Arak, Iran.

Open Access Macedonian Journal of Medical Sciences
|January 5, 2019
PubMed
Abstract

Insights

Soil samples in Arak, Iran, were analyzed for Acanthamoeba contamination. The study identified T4, T5, and T6 genotypes, with T4 being predominant, similar to other Iranian regions.

Area of Science:

  • Environmental microbiology
  • Parasitology

Background:

  • Acanthamoeba is a ubiquitous protozoan parasite.
  • Previous studies confirmed Acanthamoeba contamination in Arak's water sources.

Purpose of the Study:

  • To investigate soil as a potential reservoir for Acanthamoeba in Arak, Iran.
  • To determine the predominant Acanthamoeba genotypes in the region's soil.

Main Methods:

  • Collected and analyzed 48 soil samples from Arak, Markazi Province, Iran.
  • Employed culture-based methods and PCR assays for Acanthamoeba identification.
  • Sequenced PCR products and utilized the NCBI database for genotype determination.

Main Results:

  • Acanthamoeba contamination was detected in 33.3% of soil samples by culture and 31.25% by molecular assays.
  • The predominant genotypes identified were T4, T5, and T6.
  • Genotype T4 was the most prevalent, consistent with findings in other parts of Iran.

Conclusions:

  • Soil in Arak harbors Acanthamoeba, with T4 genotype being the most common.
  • This finding highlights the importance of soil as a potential source of Acanthamoeba in the region.
  • New sequence accession numbers (MG066681, MG298785-MG298794) were assigned for identified isolates.
Keywords:
AcanthamoebaGenotypeMolecular methodPCRSoil

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