Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

31.1K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
31.1K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.0K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.0K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

8.5K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.1K
4.1K
C4 Pathway and CAM01:27

C4 Pathway and CAM

49.1K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma proteomics based on machine learning predict the early risk of kidney outcomes in patients with DKD: a prospective cohort study from UK Biobank.

Frontiers in nephrology·2026
Same author

Curcumol Inhibits the Laminin-Facilitated Malignant Progression in Small-Cell Lung Cancer via Disrupting Laminin-Integrin β1 Interaction.

Phytotherapy research : PTR·2026
Same author

Quantitative Analysis of the Association between Clinical Antimicrobial Use and Healthcare-associated Infections: A Multicentre Case-Control Study.

The Journal of hospital infection·2026
Same author

Advances in microneedle design for the delivery of drugs, proteins, and cells in the treatment of hypertrophic scars.

Burns & trauma·2026
Same author

N-glycosylation of ERLIN2 promotes hepatocellular carcinoma progression by enhancing CCNB1 stability.

Molecular and cellular biochemistry·2026
Same author

How healthcare professionals perceive artificial intelligence risks: A grounded theory exploration of antecedents, dimensions, and outcomes.

Digital health·2026

Related Experiment Video

Updated: Jan 31, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

5.1K

The miR164-dependent regulatory pathway in developing maize seed.

Lanjie Zheng1, Xiangge Zhang1, Haojun Zhang1

  • 1College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Molecular Genetics and Genomics : MGG
|January 5, 2019
PubMed
Summary

MicroRNA164 (miR164) regulates maize seed expansion. This study identified a novel miR164 pathway involving NAC transcription factors and beta-expansin genes, crucial for seed development.

Keywords:
MaizeMiR64NAC transcription factorRegulatory pathwaySeed development

More Related Videos

Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

14.4K
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

9.2K

Related Experiment Videos

Last Updated: Jan 31, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

5.1K
Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

14.4K
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

9.2K

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Developmental Biology

Background:

  • MicroRNA164 (miR164) is vital for plant development, but its role in maize seed development is unclear.
  • Understanding miR164's function is key to maize seed development research.

Purpose of the Study:

  • To investigate the regulatory role of miR164 in maize seed development.
  • To identify target genes and pathways regulated by miR164 during seed development.

Main Methods:

  • Small RNA sequencing (sRNA-seq) and degradome sequencing.
  • RNA sequencing (RNA-seq) in transgenic lines and transient expression assays.
  • Co-expression analysis to identify gene interactions.

Main Results:

  • miR164 expression decreased during maize seed development.
  • Identified miR164 targets: NAC32 and NAC40 transcription factors.
  • Discovered a regulatory pathway: miR164-NAC32/NAC40-EXPB14/EXPB15, controlling seed expansion.

Conclusions:

  • miR164 is a significant regulator of maize seed expansion.
  • The miR164-NAC-EXPB pathway provides new insights into miRNA-mediated seed development.