Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

88.9K
Overview
88.9K
Nonlinear Pharmacokinetics: Causes of Nonlinearity01:22

Nonlinear Pharmacokinetics: Causes of Nonlinearity

725
Nonlinearity in drug pharmacokinetics is caused by various factors influencing how a drug is absorbed, distributed, metabolized, and excreted. Understanding these nonlinear processes is crucial for predicting drug behavior in the body and optimizing drug dosing regimens.
Nonlinear drug absorption can occur when the process is rate-limited by solubility, carrier-mediated transport systems, or saturation of the presystemic gut wall or hepatic metabolism. For instance, high doses of riboflavin...
725
Elasticity01:12

Elasticity

4.9K
Elasticity is the ability of an object to withstand the effects of distortion and to return to its original size and shape once the forces causing deformation are removed. When an elastic material deforms under the action of an external force, it experiences internal resistance to the deformation. However, if no external force is applied, it returns to its original state.
The elasticity of an object can be described by a stress-strain curve, which represents the relationship between stress...
4.9K
Application of Nonlinear Inequalities01:29

Application of Nonlinear Inequalities

250
A nonlinear inequality describes a comparison involving an expression that curves or behaves more complexly than a straight line. These inequalities often appear in forms that include squares, products, or variables in the denominator.To solve such an inequality, one starts by rewriting it so that zero appears on one side. For example, the inequality:  can be factored as: This form makes it easier to identify the values that cause the expression to equal zero. In this case, the...
250
Introduction to Nonlinear Inequalities01:25

Introduction to Nonlinear Inequalities

223
Linear and nonlinear inequalities are fundamental for analyzing variable relationships and identifying ranges satisfying specific conditions. A linear inequality involves variables raised only to the first power, resulting in a straight-line graph. This line partitions the coordinate plane into two distinct regions: one that satisfies the inequality and one that does not. Each region represents a set of solutions where the linear relationship holds true under the specified constraint.Nonlinear...
223
GTPases and their Regulation02:14

GTPases and their Regulation

9.8K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting tumour and organ dose in α-radiopharmaceutical therapy: a <sup>89</sup>Zr-based dosimetric approach for <sup>225</sup>Ac.

European journal of nuclear medicine and molecular imaging·2026
Same author

AARS2-mediated lactylation of ULK1 promotes autophagy-dependent progression of clear cell renal cell carcinoma.

Autophagy·2026
Same author

Sex-Dependent Determinants of Uremic Toxicity in Chronic Kidney Disease.

Toxins·2026
Same author

Histochemical characterization of TDAG51 in endothelial remodeling and angiogenesis following myocardial infarction <i>via</i> PI3K-AKT signaling.

European journal of histochemistry : EJH·2026
Same author

Transition to fetal calf serum-free culture enhances kidney proximal tubule cell bioenergetics and allows pharmacological applications.

Cell and tissue research·2026
Same author

Biomimetic MCC950-loaded cerium-layered double hydroxide nanoparticles for targeted treatment of Type 1 diabetes in streptozotocin-induced mice.

British journal of pharmacology·2026

Related Experiment Video

Updated: Jan 31, 2026

Preparation of Complaint Matrices for Quantifying Cellular Contraction
11:38

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

18.3K

Synthetic Extracellular Matrices with Nonlinear Elasticity Regulate Cellular Organization.

Kaizheng Liu1, Silvia M Mihaila2, Alan Rowan3

  • 1Radboud University , Institute for Molecules and Materials , Heyendaalseweg 135 , 6525 AJ Nijmegen , The Netherlands.

Biomacromolecules
|January 5, 2019
PubMed
Summary

Synthetic polymer hydrogels offer controlled cell culturing. Polyisocyanide (PIC) gels decouple mechanical and biological cues, showing cell responses vary with adhesive peptide density and network mechanics.

More Related Videos

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.3K
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
10:18

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

8.8K

Related Experiment Videos

Last Updated: Jan 31, 2026

Preparation of Complaint Matrices for Quantifying Cellular Contraction
11:38

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

18.3K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.3K
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
10:18

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

8.8K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Polymer Chemistry

Background:

  • Synthetic materials offer precise control over cell culture environments.
  • Polymer hydrogels are minimal synthetic biomaterials for 3D cell culturing.
  • Polyisocyanides (PIC) possess tunable macromolecular properties and nonlinear mechanics.

Purpose of the Study:

  • To investigate polyisocyanide (PIC) gels as cell culture platforms.
  • To decouple mechanical inputs from biological cues in synthetic biomaterials.
  • To understand how varying GRGDS density, polymer length, and concentration influence cell behavior.

Main Methods:

  • Encapsulating different cell types within PIC gels of tailored compositions.
  • Systematically varying adhesive peptide (GRGDS) density, polymer length, and concentration.
  • Analyzing cell proliferation, spheroid formation, and morphological changes.

Main Results:

  • Cancer and smooth muscle cells formed spheroids, proliferating based on GRGDS density, irrespective of polymer length.
  • Human adipose-derived stem cells spread, with morphology dependent on GRGDS density and network mechanics.
  • Cellular responses indicated biological cues (GRGDS density) can dominate over mechanical cues.

Conclusions:

  • PIC gels serve as effective platforms for decoupling mechanical and biological signals in cell culture.
  • Nonlinear mechanics of the extracellular matrix and its synthetic mimics are crucial for regulating cell functions.
  • Tailoring synthetic biomaterial properties can precisely control cellular behavior and tissue development.