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Generation of Mice Derived from Induced Pluripotent Stem Cells
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Generation of Mice Derived from Induced Pluripotent Stem Cells

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Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells

Natsuko F Inagaki1,2, Fuyuki F Inagaki3,4, Norihiro Kokudo3,4

  • 1Institute for Quantitative Biosciences, The University of Tokyo, Japan.

FEBS Letters
|January 5, 2019
PubMed

Insights

Researchers generated functional mouse induced pluripotent stem cell-derived mesothelial progenitor cells (miMPCs). These cells can prevent postoperative adhesion and promote liver regeneration, offering potential for cell therapy.

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Cell Therapy

Background:

  • Mesothelial cells are crucial for preventing adhesion in serous cavities.
  • Primary mesothelial progenitor cells (MPCs) have shown potential in preventing postoperative adhesion and promoting liver regeneration.
  • Induced pluripotent stem cells (iPSCs) are a promising source for regenerative medicine applications.

Purpose of the Study:

  • To establish a protocol for differentiating mouse iPSCs into functional mesothelial progenitor cells (miMPCs).
  • To evaluate the therapeutic potential of miMPCs in preventing postoperative adhesion and promoting liver regeneration.

Main Methods:

  • Development of a differentiation protocol for mouse iPSCs using defined factors.
  • Purification of iPSC-derived MPCs using specific cell surface antigens.
  • Assessment of miMPC function in preventing adhesion and promoting liver regeneration.

Main Results:

  • Successful generation of mouse iPSC-derived MPCs (miMPCs) with specific surface markers.
  • Demonstrated ability of miMPCs to suppress postoperative adhesion in preclinical models.
  • Evidence of miMPCs facilitating liver regeneration after hepatectomy.

Conclusions:

  • This study reports the first successful generation of functional miMPCs from mouse iPSCs.
  • The generated miMPCs hold significant potential for de novo cell therapy strategies.
  • These findings pave the way for novel treatments for adhesion prevention and liver regeneration.
Keywords:
cell sheetinduced pluripotent stem cellsliver regenerationmesothelial cellspostoperative adhesion

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