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Long noncoding RNA cancer susceptibility candidate 2 suppresses papillary thyroid carcinoma growth by inactivating

Fei Huang1, Qing Zhang2, Wei Chen3

  • 1Department of General Surgery, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, China.

Journal of Cellular Biochemistry
|January 5, 2019
PubMed
Abstract

Insights

Cancer susceptibility candidate 2 (CASC2) long noncoding RNA (lncRNA) suppresses papillary thyroid carcinoma (PTC) tumorigenesis. CASC2 may serve as a prognostic marker or therapeutic target for PTC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • Cancer susceptibility candidate 2 (CASC2) is a lncRNA with known tumor suppressor functions in various cancers.
  • The specific role of CASC2 in papillary thyroid carcinoma (PTC) remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of CASC2 in papillary thyroid carcinoma (PTC).
  • To determine the potential of CASC2 as a prognostic marker or therapeutic target for PTC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure CASC2 expression in PTC tissues and adjacent normal tissues.
  • Lentiviral vectors were employed to create CASC2 overexpression models in PTC cell lines.
  • Cell proliferation, apoptosis, migration, and invasion assays were performed, along with a tumor xenograft animal model study.

Main Results:

  • CASC2 expression was significantly downregulated in PTC tissues compared to normal tissues.
  • Reduced CASC2 expression correlated with larger tumor size, multifocal lesions, and advanced pathological stage.
  • Overexpression of CASC2 inhibited PTC cell proliferation, promoted apoptosis, and suppressed tumor growth in vivo.
  • CASC2 overexpression led to the inactivation of the AKT and ERK1/2 signaling pathways.

Conclusions:

  • CASC2 acts as a tumor suppressor in papillary thyroid carcinoma (PTC).
  • CASC2 warrants further investigation as a potential prognostic biomarker and therapeutic target for PTC.
Keywords:
cancer susceptibility candidate 2 (CASC2)long noncoding RNA (lncRNA)migrationpapillary thyroid carcinoma (PTC)proliferation

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