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Updated: Jan 31, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Poly(noradrenalin) based bi-enzyme biosensor for ultrasensitive multi-analyte determination.

Lanjunzi Liu1, Chao Chen1, Chenpu Chen1

  • 1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.

Talanta
|January 6, 2019
PubMed
Summary

A novel bi-enzyme biosensor was developed for simultaneous detection of glucose, hydrogen peroxide, and heavy metals like Cr(III) and Cr(VI). This biosensor offers high sensitivity and low detection limits for multi-analyte monitoring.

Keywords:
BiosensingCr(III)Cr(VI)GlucoseH(2)O(2)Oxidative polymerization of norepinephrine

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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Analytical Chemistry

Background:

  • Existing methods for physiological molecule and environmental contaminant detection often lack multi-analyte capabilities.
  • Simultaneous immobilization of enzymes like horseradish peroxidase (HRP) and glucose oxidase (GOx) is crucial for developing efficient biosensors.
  • Noradrenalin (NA) polymerization catalyzed by HRP in the presence of H2O2 offers a novel approach for enzyme immobilization.

Purpose of the Study:

  • To fabricate a novel bi-enzyme biosensor for multi-analyte detection.
  • To simultaneously immobilize HRP and GOx using HRP-catalyzed NA polymerization.
  • To evaluate the biosensor's efficiency in detecting hydrogen peroxide, glucose, and heavy metal ions (Cr(III) and Cr(VI)).

Main Methods:

  • Fabrication of a bi-enzyme biosensor by immobilizing HRP and GOx on a platinum electrode via HRP-catalyzed noradrenalin polymerization.
  • Modification of the electrode with polyaniline (PANI) to enhance performance.
  • Electrochemical characterization and performance evaluation using amperometry for detecting H2O2, glucose, Cr(III), and Cr(VI).

Main Results:

  • The PNA-HRP-GOx/Pt electrode demonstrated high sensitivity and low limits of detection for glucose (S up to 628.4 μA mM⁻¹ cm⁻², LOD 0.08 μM) and Cr(VI) (LOD 0.20 nM).
  • The PANI-modified electrode (PNA-HRP-GOx/PANI/Pt) showed linear responses to H2O2 (0.05–30.2 mM) and Cr(III) (0.01–3.8 μM).
  • The developed amperometric biosensors exhibited high sensitivity, low LOD, and good anti-interference capabilities.

Conclusions:

  • A novel and efficient bi-enzyme biosensor was successfully developed for simultaneous detection of multiple analytes.
  • The HRP-catalyzed NA polymerization method provides an effective means for simultaneous enzyme immobilization.
  • The developed biosensor holds promise for practical applications in monitoring physiological molecules and environmental contaminants.