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Raman spectroscopic method for semen identification: Azoospermia.

Marisia A Fikiet1, Igor K Lednev1

  • 1Chemistry Department, University at Albany, SUNY, 1400 Washington Ave, Albany, NY 12222, United States.

Talanta
|January 6, 2019
PubMed
Summary

Raman spectroscopy can identify semen without sperm, crucial for forensic analysis. This technique analyzes seminal fluid proteins, proving effective even in azoospermic samples.

Keywords:
AzoospermiaChemometricsForensic scienceRaman spectroscopySemenSperm

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Area of Science:

  • Forensic Science
  • Analytical Chemistry
  • Biophysics

Background:

  • Semen analysis is vital in forensic investigations, particularly in assault cases.
  • Traditional semen tests focused on sperm presence, but some semen samples lack sperm (azoospermia).
  • Current methods detect seminal fluid proteins, accommodating azoospermic samples.

Purpose of the Study:

  • To evaluate Raman spectroscopy's efficacy in identifying semen traces, especially in the absence of sperm.
  • To compare Raman spectra of semen, seminal fluid, and sperm from various donors.
  • To validate Raman spectroscopy as a tool for forensic body fluid identification.

Main Methods:

  • Raman spectroscopy was employed to analyze dry traces of semen, seminal fluid, and sperm.
  • Comparative spectral analysis was performed on samples from multiple donors.
  • A pre-existing chemometric model was used to identify spectra from seminal fluid and azoospermic semen.

Main Results:

  • The Raman spectra of semen are predominantly influenced by seminal fluid components.
  • Spectra from seminal fluid and azoospermic semen samples were accurately identified as semen using the chemometric model.
  • Analysis of semen from a vasectomized donor confirmed the dominance of seminal fluid signals.

Conclusions:

  • Sperm presence is not essential for accurate semen identification via Raman spectroscopy and chemometrics.
  • Raman spectroscopy demonstrates significant potential as a universal method for confirmatory identification of major body fluids in forensic contexts.
  • This technique offers a reliable alternative for analyzing challenging forensic samples.