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Microarray Data Analysis of Molecular Mechanism Associated with Stroke Progression

Hongmei Zhang1, Qiying Zhang2, Zuning Liao3

  • 1Department of Neurology, Fourth People's Hospital of Jinan, No. 50 Shifan Road, Tianqiao District, Jinan, 250031, Shandong Province, China.

Journal of Molecular Neuroscience : MN
|January 6, 2019
PubMed

Insights

This study identifies key molecular players in stroke, revealing miR-107-5p and androgen receptor as potential therapeutic targets for stroke treatment.

Area of Science:

  • Neuroscience
  • Genomics
  • Bioinformatics

Background:

  • Stroke is a leading cause of death and disability worldwide.
  • Understanding the molecular mechanisms underlying stroke is crucial for developing effective treatments.

Purpose of the Study:

  • To explore the molecular mechanisms of stroke using bioinformatics.
  • To identify novel molecular targets for clinical management of stroke.

Main Methods:

  • Analysis of miRNA (GSE97532) and mRNA (GSE97533) datasets from middle cerebral artery occlusion (MCAO) and sham operation models.
  • Screening for differentially expressed genes (DEGs) and miRNAs (DEMIRs).
  • Exploration of DEMIR-DEG interactions, pathway enrichment, protein-protein interaction (PPI) network construction, and transcription factor (TF) analysis.

Main Results:

  • Identified 38 DEMIRs and 546 DEGs between MCAO and sham groups.
  • Revealed 370 DEMIR-DEG interactions, including miR-107-5p-Furin.
  • Top DEMIRs enriched in cAMP signaling pathway; target DEGs involved in ATP binding and ABC transporters.
  • Identified androgen receptor (AR) as a key TF.

Conclusions:

  • miR-107-5p may influence stroke progression by inhibiting Furin expression.
  • AR and cAMP signaling pathway represent potential novel therapeutic targets for stroke.
  • ATP binding functions may offer new avenues for stroke treatment.
Keywords:
Differentially expressed mRNADifferentially expressed miRNAFunction and pathway analysisStrokeTranscription factors

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