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Profiling Activity of Cellular Kinases in Migrating T-Cells

Chandra Sekhar Chirumamilla1, Mobashar Hussain Urf Turabe Fazil2, Claudina Perez-Novo1

  • 1Laboratory of Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Antwerpen, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2019
PubMed

Insights

T-Lymphocyte kinases regulate T-cell movement. A new phosphopeptide array method identifies hyper-activated kinases controlling T-cell migration, offering insights into T-cell status.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • T-Lymphocyte kinases are crucial for T-cell motility, regulating signal transduction pathways.
  • Understanding T-cell kinome dynamics is vital for characterizing T-cell states.
  • Current methods for kinase characterization are limited in scope or dynamic range.

Purpose of the Study:

  • To introduce a novel multiplex phosphopeptide array methodology.
  • To enable kinome-wide identification of hyper-activated kinases in motile T-cells.
  • To explore kinase activity patterns as biomarkers for T-cell status.

Main Methods:

  • Development and application of a multiplex phosphopeptide array.
  • Analysis of kinase activation states in T-cells.
  • Focus on identifying kinases regulating T-cell migration.

Main Results:

  • The phosphopeptide array successfully identified hyper-activated kinases.
  • The method allows for kinome-wide screening of kinase activity.
  • Specific kinase profiles correlate with T-cell migration.

Conclusions:

  • Multiplex phosphopeptide arrays offer a powerful tool for kinome-wide analysis.
  • This methodology can identify key kinases regulating T-cell migration.
  • Kinase fingerprinting may serve as a diagnostic indicator for T-cell function.
Keywords:
ICAM-1KinaseKinome analysisLFA-1Peptide microarrayT-cell

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