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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
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Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
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Updated: Jan 31, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Creating Large Chromosomal Deletions in Rice Using CRISPR/Cas9.

Riqing Li1, Si Nian Char1, Bing Yang2

  • 1Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2019
PubMed
Summary

This study details a CRISPR/Cas9 protocol for creating large chromosomal deletions in rice. This genome engineering technique aids in crop improvement and genetic analysis.

Keywords:
Agrobacterium-mediated rice transformationCRISPR/Cas9Genome editingLarge chromosomal deletionRiceTargeted mutagenesis

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Area of Science:

  • Plant science
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a widely used genome engineering tool in plants.
  • It enables precise genetic modifications for crop improvement, including yield and stress resistance.
  • CRISPR/Cas9 is effective for generating large chromosomal deletions in plants.

Purpose of the Study:

  • To present a detailed protocol for creating large chromosomal deletions in rice using the CRISPR/Cas9 system.
  • To facilitate genetic analysis and functional studies of gene clusters in rice.

Main Methods:

  • Single-guide RNA design specific for target chromosomal regions.
  • Vector construction for CRISPR/Cas9 delivery.
  • Rice plant transformation and regeneration.
  • Screening methods for identifying large deletions.

Main Results:

  • Successful generation of large chromosomal deletions in rice plants.
  • Demonstration of the protocol's efficacy from design to screening.
  • Validation of CRISPR/Cas9 for targeted chromosomal engineering in a major crop.

Conclusions:

  • The presented protocol offers a robust method for creating large chromosomal deletions in rice.
  • This technique is valuable for advancing genetic research and crop breeding.
  • CRISPR/Cas9-mediated deletions provide insights into plant genome organization and function.