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Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
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Genome Editing in Citrus Tree with CRISPR/Cas9.
Hongge Jia1, Xiuping Zou1, Vladimir Orbovic2
1Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2019
Summary
This study modified the citrus gene CsLOB1 using CRISPR/Cas9 genome editing technology. The research demonstrates successful gene editing in transgenic citrus plants created via Agrobacterium-mediated transformation.
Area of Science:
- Plant biotechnology
- Genomics
- Molecular biology
Background:
- CRISPR/Cas9 is a versatile genome editing tool used across diverse organisms.
- Modifying specific genes in plants is crucial for agricultural advancements.
Purpose of the Study:
- To describe the modification of the citrus gene CsLOB1 using the CRISPR/Cas9 system.
- To establish a method for creating transgenic citrus plants with targeted gene edits.
Main Methods:
- Utilized the Cas9/sgRNA two-component system derived from CRISPR-Cas9.
- Employed Agrobacterium-mediated epicotyl transformation to generate transgenic citrus plants.
Main Results:
- Successfully modified the citrus gene CsLOB1 in transgenic plants.
- Demonstrated the efficacy of CRISPR/Cas9 for targeted gene editing in citrus.
Conclusions:
- CRISPR/Cas9 is an effective tool for citrus gene editing.
- Agrobacterium-mediated transformation is a viable method for producing edited transgenic citrus.
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