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Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1
Xiaojia Yin1, Abhishek Anand1, Paul Quick1
1International Rice Research Institute, Manila, Philippines.
Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2019
Summary
The CRISPR/Cpf1 (Cpf1) genome editing tool efficiently modified the OsEPFL9 gene in rice, impacting leaf stomatal density. This advancement offers new insights into plant physiology and water use efficiency under stress.
Area of Science:
- Plant Biology
- Genetics
- Molecular Biology
Background:
- CRISPR/Cas9 (Cas9) is a widely used genome editing tool but has limitations.
- CRISPR/Cpf1 (Cpf1) offers an alternative with distinct advantages for genome editing.
Purpose of the Study:
- To evaluate the efficiency of the Cpf1 system in rice.
- To investigate the role of the OsEPFL9 gene in regulating stomatal density.
- To explore Cpf1's potential for improving plant water use efficiency.
Main Methods:
- Utilized CRISPR/Cpf1 (Cpf1) for targeted genome editing in rice.
- Focused editing on Exon1 of the OsEPFL9 gene.
- Analyzed knockout lines over multiple generations for edit stability and inheritance.
Main Results:
- Successfully demonstrated Cpf1 system efficiency in rice.
- Confirmed Cpf1's ability to create stable genetic modifications in OsEPFL9.
- Observed alterations in stomatal density, indicating OsEPFL9's regulatory role.
Conclusions:
- CRISPR/Cpf1 (Cpf1) is an effective tool for manipulating plant genes like OsEPFL9.
- Genome editing of OsEPFL9 can influence stomatal patterning and potentially enhance water use efficiency.
- Further research using Cpf1 can provide deeper understanding of rice physiology under stress.
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