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Updated: Jan 31, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
Published on: January 9, 2019
Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System
Yang Chen1, Qian Peter Su2, Li Yu3
1Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University, Beijing, China.
Abstract:
Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.
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