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Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
Published on: May 3, 2013
Characterization of the "Autophagic Flux" in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and
D Kalamida1, A Giatromanolaki2, M I Koukourakis3
1Department of Radiotherapy/Oncology, Democritus University of Thrace, and University General Hospital of Alexandroupolis, Alexandroupolis, Greece.
Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2019
Abstract:
A method is described for the immunostaining of human prostate cancer biopsies for the autophagic marker LC3 and the lysosomal protein LAMP2A. Using this combination we can provide some information on autophagic flux, and specific patterns of staining have been recognized for normal prostate tissue and prostatic carcinomas.
