Related Experiment Video
Updated: Jan 31, 2026
Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
Published on: May 31, 2024
Isolation of clinically relevant concentrations of bone marrow mesenchymal stem cells without centrifugation
Michael Scarpone1, Daniel Kuebler2, Andrew Chambers3
1Trinity Sports Medicine and Performance Center, Trinity Hospital, Steubenville, OH, 43952, USA. scarponesportsmedicine@trinityhealth.com.
Background:
This study examined the quality of bone marrow aspirates extracted using a novel, FDA cleared method to optimally target cells from the inner cortical iliac bone surface without the need for centrifugation. This method employs small draws from a single puncture that promote only lateral flow from multiple sites (SSLM method). The study utilized the Marrow Cellutions bone marrow aspiration system (MC system) which is based on the SSLM method and compared the MC system directly to bone marrow concentrates (BMAC) generated by centrifugation of aspirates harvested with a standard aspiration needle.
Methods:
Three direct comparisons were conducted evaluating the SSLM draws and BMACs derived from the same patient from contralateral iliac crests. The levels of TNCs/mL, CD34+ cells/mL, CD117+ cells/mL, and CFU-f/mL were compared between the various bone marrow preparations. The cellular content of a series of SSLM draws was also analyzed to determine the total nucleated cell (TNC) count and the concentration of mesenchymal stem/progenitor cells as measured by colony forming unit fibroblasts (CFU-f).
Results:
In direct comparisons with BMAC systems, SSLM draws yielded significantly higher CFU-f concentrations and comparable concentrations of CD34+ and CD117+ cells. In addition, the average quantity of TNCs/mL in a series of 30 patients utilizing the SSLM draw was 35.2 × 106 ± 17.1 × 106 and the average number of CFU-f/mL was 2885 ± 1716. There were small but significant correlations between the TNCs/mL and the CFU-fs/mL using the SSLM method as well as between the age of the patient and the CFU-fs/mL.
Conclusions:
The MC Device, using the SSLM draw technique, produced concentrations of CFU-fs, CD34+ cells and CD117+ cells that were comparable or greater to BMACs derived from the same patient. Given the rapid speed and simplicity of the MC Device, we believe this novel system possesses significant practical advantages to other currently available centrifugation based systems.
Insights
The novel Marrow Cellutions (MC) system using small lateral draws (SSLM) method yields higher concentrations of colony-forming unit fibroblasts (CFU-f) and comparable stem cell yields to traditional bone marrow aspirate concentrates (BMAC). This offers a simpler, faster alternative for bone marrow cell collection.
Area of Science:
- Regenerative Medicine
- Hematology
- Biotechnology
Background:
- A novel FDA-cleared method, the small draws from a single puncture that promote only lateral flow from multiple sites (SSLM) method, was developed for bone marrow aspiration.
- This technique targets cells from the inner cortical iliac bone surface, aiming to improve aspirate quality without centrifugation.
Purpose of the Study:
- To evaluate the cellular quality of bone marrow aspirates obtained using the Marrow Cellutions (MC) system, which employs the SSLM method.
- To compare the MC system's performance directly against bone marrow aspirate concentrates (BMAC) generated by standard centrifugation methods.
Main Methods:
- Three direct comparative studies were performed using contralateral iliac crests from the same patients.
- Key cellular components, including total nucleated cells (TNCs), CD34+ cells, CD117+ cells, and colony-forming unit fibroblasts (CFU-f), were quantified per milliliter.
- The study also analyzed the cellular content of serial SSLM draws to determine TNC counts and mesenchymal stem/progenitor cell concentrations (CFU-f).
Main Results:
- SSLM draws demonstrated significantly higher CFU-f concentrations compared to BMAC.
- Concentrations of CD34+ and CD117+ cells were comparable between SSLM draws and BMAC.
- The average TNC concentration in 30 patients using SSLM was 35.2 × 10^6 ± 17.1 × 10^6 cells/mL, with an average CFU-f concentration of 2885 ± 1716 cells/mL.
Conclusions:
- The MC Device, utilizing the SSLM technique, yields bone marrow preparations with CFU-f concentrations comparable to or greater than BMAC.
- The study highlights the MC Device's potential for producing high-quality stem cell yields.
- The SSLM method offers significant practical advantages in speed and simplicity over current centrifugation-based systems for bone marrow aspiration.
Related Concept Videos
01:19Mesenchymal Stem Cells
01:22Bone Marrow Sampling and Transplants
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
01:19Clinical Applications of Epidermal Stem Cells
02:41Concentration Cells
Consider the following voltaic cell:
01:29Clinically Relevant Drug Product Specifications: Methods of Establishment
01:05Centrifugation