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Evaluating the preservation and isolation of stool pathogens using the COPAN FecalSwab™ Transport System and

Lee W Goneau1, Anthony Mazzulli2, Xhermi Trimi2

  • 1Public Health Ontario, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada.

Diagnostic Microbiology and Infectious Disease
|January 7, 2019
PubMed

Insights

This study shows that the automated COPAN WASP® system with FecalSwab™ and selenite media improves enteric pathogen isolation from stool. This method enhances pathogen recovery and colony isolation compared to standard techniques.

Area of Science:

  • Clinical Microbiology
  • Diagnostic Microbiology
  • Bacteriology

Background:

  • Isolation of enteric pathogens from stool is challenging due to fastidious organisms and flora overgrowth.
  • Standard methods for stool specimen processing can limit pathogen recovery and accurate diagnosis.

Purpose of the Study:

  • To evaluate an automated system for improved stool pathogen preservation and isolation.
  • To compare the efficacy of FecalSwab™ and selenite media with standard methods.

Main Methods:

  • Utilized the COPAN Walk-Away Specimen Processor (WASP®) with FecalSwab™ and selenite media.
  • Assessed pathogen viability and commensal abundance under different storage conditions.
  • Compared WASP® planting to the Isoplater system for colony isolation.

Main Results:

  • FecalSwab™ maintained pathogen viability and stable commensal abundance at room temperature and refrigerated conditions.
  • Significantly increased well-isolated pathogen colonies were observed compared to modified Cary-Blair media.
  • WASP® planting improved individual pathogen colony isolation over the Isoplater system.
  • COPAN selenite media enhanced Salmonella enterica serovar Typhimurium yield.

Conclusions:

  • The automated WASP® system, FecalSwab™, and selenite media offer a rapid and efficient approach for stool specimen processing.
  • This integrated system improves enteric pathogen isolation and diagnostic yield compared to conventional methods.
Keywords:
Cary–BlairEnteric pathogenFecalSwabStool cultureWASP

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