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Use of human alpha-satellite deoxyribonucleic acid to detect Y-specific centromeric sequences
S P Tho1, A Behzadian, J R Byrd
1Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
American Journal of Obstetrics and Gynecology
|December 1, 1988
Summary
The Y97 alphoid DNA probe accurately identifies the human Y centromere and Y-specific fragments. This highly sensitive and specific probe is useful for detecting Y chromosome alterations, even in mosaic cell lines.
Area of Science:
- Genetics
- Molecular Biology
- Human Cytogenetics
Background:
- The human Y chromosome contains unique alphoid DNA sequences.
- Specific probes are needed to accurately map and identify Y chromosome components.
Purpose of the Study:
- To evaluate the sensitivity and specificity of the Y97 alphoid DNA probe.
- To confirm the Y97 probe's utility in identifying Y chromosome alterations.
Main Methods:
- Hybridization experiments using the Y97 probe on male and female DNA mixtures.
- Comparative analysis with the Yq11-related probe 4B-2.
- Testing the Y97 probe on subjects with mosaic cell lines containing Y fragments.
Main Results:
- The Y97 probe specifically detected the Y-specific 5.5 kb Eco RI fragment in a 1% male DNA mixture.
- Nineteen out of 20 subjects with mosaic cell lines showed a positive hybridization band with the Y97 probe.
- The probe confirmed the presence of Y-specific fragments in cytogenetically altered Y chromosomes.
Conclusions:
- The Y97 probe is a sensitive and specific tool for identifying the human Y centromere.
- This probe is effective in detecting Y chromosome alterations and mosaicism.
- Alphoid repeat sequences are confirmed to be located in the centromeric region of the Y chromosome.