Related Experiment Video

Updated: Jan 31, 2026

In Vivo Vascular Permeability Detection in Mouse Submandibular Gland
07:10

In Vivo Vascular Permeability Detection in Mouse Submandibular Gland

Published on: August 4, 2022

2.5K

Vascular Permeability Assay in Human Coronary and Mouse Brachiocephalic Arteries

Liang Guo1, Raquel Fernandez1, Atsushi Sakamoto1

  • 1CVPath Institute, Gaithersburg, Maryland, USA.

Bio-Protocol
|January 8, 2019
PubMed

Insights

This study establishes a novel method using Evans Blue Dye to measure microvascular permeability in human coronary arteries. This technique aids in understanding coronary artery disease progression and endothelial integrity.

Area of Science:

  • Cardiovascular Science
  • Pathology
  • Histology

Background:

  • Coronary artery disease (CAD) is a major cause of death.
  • Intraplaque hemorrhage, driven by microvessel angiogenesis and increased vascular permeability, contributes to CAD progression.
  • Current methods lack established protocols for assessing microvascular permeability in human coronary arteries.

Purpose of the Study:

  • To establish a protocol for evaluating microvascular permeability and angiogenesis in fresh human coronary artery autopsy samples.
  • To adapt the Evans Blue Dye (EBD) assay for human coronary artery research.
  • To provide a functional measure of endothelial integrity in CAD.

Main Methods:

  • Detailed protocol for perfusing human coronary arteries.
  • Sample collection for histological analysis and immunostaining.
  • Image analysis methodology for microvascular permeability assessment.
  • Optional FITC-dextran assay in a mouse model for vascular permeability evaluation.

Main Results:

  • The described protocol enables the evaluation of microvascular permeability in human coronary artery samples.
  • The EBD assay can be adapted for functional assessment of endothelium integrity.
  • The method provides insights into pathological processes in CAD.

Conclusions:

  • The established Evans Blue Dye protocol is a valuable tool for assessing microvascular permeability in human coronary arteries.
  • This method can be applied to both human and animal models for studying vascular permeability in various diseases.
  • The protocol offers a functional measure of endothelial integrity relevant to coronary artery disease research.

Keywords:
AngiogenesisAtherosclerosisCoronary artery diseaseEvans blue dyeFITC-dextranIntraplaque hemorrhageMicrovessel permeability

More Related Videos

Isolation of Murine Coronary Vascular Smooth Muscle Cells08:24

Isolation of Murine Coronary Vascular Smooth Muscle Cells

Published on: May 30, 2016

14.8K
Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA13:10

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA

Published on: April 24, 2017

19.2K

Related Concept Videos

Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
1.1K
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
428
Coronary Artery Disease V: Interprofessional Care01:27

Coronary Artery Disease V: Interprofessional Care

Interprofessional care for coronary artery disease includes pharmacological therapy and revascularization procedures.Pharmacological therapy for Coronary Artery Disease (CAD) aims to manage symptoms, prevent complications, and improve patient outcomes through various classes of medications:Antiplatelet Agents:Aspirin and Clopidogrel: These medications inhibit platelet aggregation, preventing blood clots, which is crucial for avoiding heart attacks and strokes. Doctors often prescribe these...
267
Coronary Artery Disease III: Clinical Manifestations01:30

Coronary Artery Disease III: Clinical Manifestations

Coronary Artery Disease (CAD) is a primary health risk worldwide, leading to significant morbidity and mortality. The condition arises from the buildup of atherosclerotic plaques within the coronary arteries, resulting in diminished blood supply to the heart muscle.The clinical manifestations of CAD vary widely, from asymptomatic stages to severe, life-threatening conditions. Understanding these manifestations is crucial for early diagnosis and effective management.Angina Pectoris: The Warning...
369
Coronary Artery Disease IV: Preventive Measures01:26

Coronary Artery Disease IV: Preventive Measures

Effective preventive measures for coronary artery disease (CAD) focus on controlling modifiable risk factors, including cholesterol abnormalities and lifestyle changes.Cholesterol ManagementFirst, the Mediterranean diet and the American Heart Association advocate for maintaining low-density lipoprotein (LDL) cholesterol levels below 100 mg/dL, with a more stringent recommendation of below 70 mg/dL for individuals at high risk. LDL cholesterol, often termed "bad cholesterol," can lead to the...
686
Permeability of Concrete01:25

Permeability of Concrete

Permeability in the context of concrete refers to how easily liquids or gases can pass through the material. This quality is crucial for assessing the water-tightness and durability of concrete structures and their resistance to chemical attacks. Concrete permeability can be determined through comparative laboratory tests. These tests typically involve sealing a concrete specimen from the sides, applying water pressure to the top surface with pressure, and measuring the amount of water passing...
505