Related Experiment Video

Updated: Jan 31, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

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MDA-19 Suppresses Progression of Melanoma Via Inhibiting the PI3K/Akt Pathway

Ningning Dang1, Xianguang Meng1, Shanshan Ma1

  • 1Department of Dermatology, Jinan Central Hospital affiliated to Shandong University, Jinan 250013, Shandong Province, P.R. China.

Open Medicine (Warsaw, Poland)
|January 8, 2019
PubMed
Abstract

Insights

MDA-19 effectively inhibits melanoma progression by reducing cell growth, migration, and invasion. This compound also promotes apoptosis and suppresses the PI3K/Akt pathway, indicating its potential as a melanoma therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Melanoma is an aggressive skin cancer with limited treatment options.
  • Understanding novel therapeutic targets is crucial for effective melanoma treatment.

Purpose of the Study:

  • To investigate the anti-melanoma effects of MDA-19.
  • To elucidate the underlying molecular mechanisms of MDA-19 action.

Main Methods:

  • Melanoma cell lines (M14, UACC257) were treated with MDA-19.
  • Assays included CCK8, clone formation, Transwell, and flow cytometry.
  • Protein expression of apoptosis, EMT, and signaling pathway markers was analyzed via Western blot.

Main Results:

  • MDA-19 demonstrated dose-dependent inhibition of melanoma cell proliferation, migration, and invasion.
  • Apoptosis was induced via the mitochondrial pathway, regulating Bcl-2/Bax and Caspase 3.
  • MDA-19 modulated epithelial-mesenchymal transition (EMT) markers (E-cad, N-cad, Vimentin, Slug).
  • The PI3K/Akt pathway was inhibited by blocking Akt phosphorylation and downstream targets (P70, Cyclin D1).

Conclusions:

  • MDA-19 inhibits melanoma progression by suppressing the PI3K/Akt signaling pathway.
  • MDA-19 shows potential as an anti-cancer agent for melanoma therapy.
Keywords:
EMTMDA-19PI3K/Aktprogression

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