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Updated: Jan 31, 2026
A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model
Published on: November 18, 2022
Circular RNA microarray expression profile in 3,4-benzopyrene/angiotensin II-induced abdominal aortic aneurysm in
Jiaoni Wang1,2, Huankun Sun1, Yingying Zhou3
1Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.
Abstract:
Abdominal aortic aneurysm (AAA) is an unpredictable but lethal disease that poses a therapeutic dilemma. Circular RNAs (circRNAs), whose functional roles as transcriptional regulators and microRNA (miRNA) sponges have been shown in former studies, are potential biomarkers for many diseases. AAA in male C57BL/6 J mice was induced by coadministration of angiotensin II (Ang II) and 3,4-benzopyrene (BaP). The circRNA expression profiling was performed using two samples from the control group and two samples from the AAA group. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to confirm the reliability of the microarray results. Among the 14 236 detected circRNAs, 413 showed obvious expression changes (fold change ≥ 2; P < 0.05) between the BaP/Ang II-induced AAA group and control group. Of the 413 that showed significant changes, 271 were upregulated, while the other 142 were downregulated. The expression levels of 10 circRNAs were validated by qRT-PCR. The interactions of the differentially expressed circRNAs with miRNAs were predicted. Immunofluorescence showed prominent vascular smooth muscle cell apoptosis in abdominal aortic tissues in the BaP/Ang II group. Furthermore, a circRNA-miRNA coexpression network based on six apoptosis-related circRNAs was built. The genes regulated by the network mapped to several pathways, including apoptosis, the IL-17 signaling pathway, and vascular endothelial growth factor signaling pathway, all of which are related to AAA formation. This study performed circRNA expression profiling in AAA and the results specifically predicted the regulatory role of circRNAs in AAA pathogenesis.
Insights
Circular RNAs (circRNAs) show altered expression in abdominal aortic aneurysm (AAA). These circRNAs may regulate AAA development by influencing apoptosis and related signaling pathways, offering potential diagnostic biomarkers.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Genomics
Background:
- Abdominal aortic aneurysm (AAA) is a life-threatening condition with limited therapeutic options.
- Circular RNAs (circRNAs) are emerging as key regulators in various diseases and potential biomarkers.
- The role of circRNAs in AAA pathogenesis remains largely unexplored.
Purpose of the Study:
- To investigate circRNA expression profiles in a mouse model of abdominal aortic aneurysm (AAA).
- To identify differentially expressed circRNAs and predict their interactions with microRNAs (miRNAs).
- To elucidate the potential role of circRNAs in AAA development and identify novel therapeutic targets.
Main Methods:
- AAA was induced in male C57BL/6J mice using angiotensin II (Ang II) and 3,4-benzopyrene (BaP).
- CircRNA expression profiling was performed using microarray analysis on AAA and control samples.
- Quantitative real-time polymerase chain reaction (qRT-PCR) was used for validation, and bioinformatics tools predicted circRNA-miRNA interactions.
Main Results:
- A total of 14,236 circRNAs were detected, with 413 showing significant differential expression (fold change ≥ 2; P < 0.05) between AAA and control groups.
- Of these, 271 circRNAs were upregulated and 142 were downregulated in the AAA group.
- A circRNA-miRNA coexpression network involving six apoptosis-related circRNAs was constructed, implicating pathways like apoptosis and IL-17 signaling in AAA.
Conclusions:
- CircRNA expression is significantly altered in abdominal aortic aneurysm.
- Differentially expressed circRNAs, particularly those involved in apoptosis, may play a crucial role in AAA pathogenesis.
- These findings highlight circRNAs as potential diagnostic biomarkers and therapeutic targets for AAA.
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