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Probiotic characterization of Lactobacillus isolates from canine faeces
M M Coman1, M C Verdenelli1, C Cecchini1
1Synbiotec S.r.l., Camerino, Italy.
Journal of Applied Microbiology
|January 8, 2019
Summary
Lactobacillus reuteri AI, a dog-derived probiotic, effectively combats canine intestinal infections. This selected strain demonstrates significant antimicrobial properties and survival capabilities, making it a promising dietary supplement for canine health.
Area of Science:
- Veterinary Microbiology
- Animal Nutrition
- Gastroenterology
Background:
- Canine intestinal infections are a growing concern.
- Autochthonous probiotic bacteria offer a potential solution for gut health in dogs.
- Lactobacillus species of canine origin are of particular interest for host-specific probiotic applications.
Purpose of the Study:
- To isolate and characterize canine intestinal Lactobacillus strains.
- To select specific probiotic candidates for use as dietary supplements in dogs.
- To promote the health status of dogs through targeted gut microbial interventions.
Main Methods:
- Isolation and screening of Lactobacillus strains from dog fecal samples.
- Evaluation of probiotic properties: survival to gastric/pancreatic juices and bile salts, antibiotic resistance, antipathogenicity.
- Molecular identification (16S rRNA gene sequencing) and in vitro verification (sugar fermentation, HT29 cell adhesion).
- Assessment of freeze-drying resistance and in vivo administration for strain permanence.
Main Results:
- Fourteen Lactobacillus strains were isolated; five showed tolerance to gastric stress.
- Three strains identified as Lactobacillus reuteri and two as Lactobacillus johnsonii.
- Lactobacillus reuteri AI demonstrated significant antimicrobial capacity and resistance to clindamycin.
- L. reuteri AI survived freeze-drying and showed in vivo persistence (26.7% recovery post-administration).
Conclusions:
- Lactobacillus reuteri AI is the most promising probiotic candidate among the selected strains for canine dietary supplements.
- The selection of autochthonous probiotic strains is a valuable strategy for managing canine gut health issues.
- This research supports the use of specific Lactobacillus strains to control intestinal pathogens in dogs.
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