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Updated: Jan 31, 2026
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
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Clinically useful flow cytometry approach to identify immunophenotype in acute leukemia.
Guifang Ouyang1,2, Zhijuan Xu1,2, Danjie Jiang3,2
11 Department of Hematology, Ningbo First Hospital, Ningbo, Zhejiang, China.
The Journal of International Medical Research
|January 8, 2019
Summary
Flow cytometry rapidly identifies immunophenotypes in acute leukemia (AL). The study suggests cluster of differentiation 2 (CD2) may predict prognosis in acute myeloid leukemia (AML) patients.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Acute leukemia (AL) is a complex malignant disease originating from hematopoietic cell abnormalities.
- Immunophenotyping is crucial for AL diagnosis and classification.
- Flow cytometry offers a potential method for rapid immunophenotyping.
Purpose of the Study:
- To investigate the utility of flow cytometry for immunophenotyping leukemic cells.
- To assess the clinical usefulness of immunophenotyping in AL treatment.
- To explore the prognostic value of specific immunophenotypic markers.
Main Methods:
- Bone marrow specimens from AL patients were analyzed for antigen expression using flow cytometry.
- Kaplan-Meier survival analysis was employed to evaluate marker impact on prognosis.
- Genetic testing was performed to detect the BCR-ABL fusion gene.
Main Results:
- Distinct immunophenotypic marker expression profiles were observed between acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML).
- A significant association between the CD2 surface marker and survival was found in AML, but not in ALL.
- Increased prevalence of the BCR-ABL fusion gene was noted in ALL compared to AML samples.
Conclusions:
- Flow cytometry provides a rapid and effective method for AL immunophenotype identification.
- The CD2 marker shows potential as a predictive prognostic indicator for AML patients.
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