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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Linear momentum is a fundamental concept in physics that describes the motion of an object. It is a vector quantity, having a magnitude equal to the product of its mass and its velocity, and direction along the object's velocity. On the other hand, linear impulse, also known as momentum impulse, is a concept in physics related to the change in the linear momentum of an object. Impulse is a vector quantity defined as the product of force and the time over which the force is applied.
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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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Updated: Jan 31, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Linearization and Labeling of Single-Stranded DNA for Optical Sequence Analysis.

Rajib Basak1, Fan Liu1, Sarfraz Qureshi1

  • 1Department of Physics , National University of Singapore , Singapore 117542.

The Journal of Physical Chemistry Letters
|January 8, 2019
PubMed
Summary
This summary is machine-generated.

Controlled polypeptide coating linearizes single-stranded DNA (ssDNA), preventing self-annealing for improved genetic profiling. This enzyme-free method enables site-specific labeling and sequence detection.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Single-stranded DNA (ssDNA) is highly flexible and prone to self-annealing, hindering its use in genetic profiling.
  • Linearization of ssDNA is crucial for exposing unpaired bases to gene-targeting probes.

Purpose of the Study:

  • To develop a method for controlled linearization of ssDNA.
  • To prevent ssDNA self-annealing during genetic analysis.
  • To enable enzyme-free, site-specific labeling of ssDNA.

Main Methods:

  • Coating ssDNA with a cationic-neutral diblock polypeptide copolymer.
  • Linearizing coated ssDNA using nanochannel confinement or molecular combing.
  • Analyzing ssDNA linearization and structure using atomic force microscopy.
  • Confirming site-specific binding of labeled oligonucleotides.

Main Results:

  • Polypeptide coating effectively prevents ssDNA self-annealing.
  • Nanochannel confinement and molecular combing achieve significant ssDNA stretching (0.32 nm/nucleotide).
  • Coated ssDNA exhibits contour and persistence lengths similar to B-form double-stranded DNA (dsDNA).
  • Site-specific binding of labeled oligonucleotides is preserved.

Conclusions:

  • Controlled polypeptide coating is a viable strategy to linearize ssDNA.
  • This method facilitates enzyme-free, sequence-specific labeling of ssDNA.
  • The approach enhances opportunities for genetic profiling and sequence detection.