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Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging.

Maysamreza Chamanzar1,2,3, David J Garfield4,5,3, Jillian Iafrati6

  • 1Electrical and Computer Engineering Department, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Biomedical Optics Express
|January 8, 2019
PubMed
Summary

Researchers developed micro-structured lightbulbs (MLBs) using upconverting nanoparticles (UCNPs) for deep-tissue optical stimulation and imaging. These UCNPs enable near-infrared light delivery for optogenetics and tracking in biological tissues.

Keywords:
(130.3990) Micro-optical devices(170.0110) Imaging systems(190.7220) Upconversion

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Area of Science:

  • Biomedical Optics
  • Nanotechnology
  • Neuroscience

Background:

  • Visible light optical methods face limitations in deep tissue penetration due to light absorption and scattering.
  • Optogenetics and deep-tissue imaging require efficient light delivery systems for biological applications.

Purpose of the Study:

  • To design and implement passive micro-structured lightbulbs (MLBs) for deep-tissue light delivery.
  • To utilize lanthanide-doped upconverting nanoparticles (UCNPs) for optical stimulation and imaging within biological tissues.

Main Methods:

  • Fabrication of Parylene C cylindrical pillars containing bright-emitting lanthanide-doped upconverting nanoparticles (UCNPs).
  • Encapsulation of UCNPs within MLBs for implantation deep into tissue.
  • Utilizing 980 nm near-infrared (NIR) light absorption by UCNPs for local blue light emission.

Main Results:

  • MLBs successfully delivered blue light (via 1G4→3H6 and 1D2→3F4 transitions) for potential optogenetic excitation of neurons.
  • UCNPs emitted higher energy NIR photons at 800 nm (via 3H4→3H6 transition) for deep-tissue imaging and MLB tracking.
  • Demonstrated efficient light delivery deep into tissue, overcoming limitations of visible light.

Conclusions:

  • Passive MLBs with UCNPs offer a novel approach for deep-tissue optical applications.
  • This technology enables both neuronal stimulation and non-invasive tracking of implanted devices.
  • Overcomes significant scattering and absorption challenges inherent in deep-tissue optical interventions.