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Proteolytic and nematicidal potential of the compost colonized by Hypsizygus marmoreus

Filippe Elias de Freitas Soares1, Vânia Mayumi Nakajima2, Bruna Leite Sufiate2

  • 1Federal University of Viçosa, Biochemistry and Molecular Biology Department, Viçosa, Minas Gerais, Cep: 3657000, Brazil; State University of Minas Gerais, Campus Ubá, Av. Olegário Maciel, 1427, Ubá, Minas Gerais, Cep: 36500-000, Brazil.

Experimental Parasitology
|January 8, 2019
PubMed

Insights

Spent mushroom compost (SMC) from Hypsizygus marmoreus contains proteases that effectively reduce nematode larvae. This discovery offers a potential biological control for plant-parasitic nematodes.

Area of Science:

  • Biotechnology
  • Biochemistry
  • Agricultural Science

Background:

  • Spent mushroom compost (SMC) is a byproduct of edible mushroom cultivation.
  • Hypsizygus marmoreus genome sequencing revealed cuticle-degrading protease genes.
  • Nematode infections pose significant threats to agriculture.

Purpose of the Study:

  • To investigate and characterize proteases from H. marmoreus SMC.
  • To evaluate the nematicidal activity of these proteases against nematode larvae.

Main Methods:

  • Water extraction of proteases from H. marmoreus SMC.
  • Proteolytic activity assay.
  • Zymogram and SDS-PAGE for protease identification and molecular weight determination.
  • Nematicidal assays on Panagrellus redivivus and L3 infective larvae.

Main Results:

  • Efficient extraction of proteases from SMC with high proteolytic activity (195.36 U g⁻¹).
  • Identification of two proteases (30.2 and 33.7 kDa) with potential cuticle-degrading activity.
  • Significant reduction in nematode larvae: 52% for P. redivivus and 26% for L3.

Conclusions:

  • H. marmoreus SMC is a viable source of easily extractable proteases.
  • These proteases exhibit significant nematicidal effects, indicating potential for biological control applications.
  • Further research into cuticle-degrading enzymes could lead to novel nematicides.
Keywords:
Hypsizygus marmoreusIn silicoNematicidalProteaseSpent mushroom compost

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