Deep Vein Thrombosis is Modulated by Inflammation Regulated via Sirtuin 1/NF-κB Signalling Pathway in a Rat Model.

Area of Science:

• Vascular Biology
• Inflammation Research
• Thrombosis Studies

Background:

• Inflammation is crucial in thrombus formation.
• Sirtuin 1 (SIRT1) negatively regulates inflammation by deacetylating nuclear factor-kappa B.
• The role of SIRT1-regulated inflammation in deep vein thrombosis (DVT) remains unclear.

Purpose of the Study:

• To investigate the role of SIRT1 in inferior vena cava (IVC) stenosis-induced DVT.
• To determine if SIRT1 activation can attenuate DVT.

Main Methods:

• Rats underwent IVC stenosis to induce DVT.
• Thrombus weight and histopathology were analyzed.
• Serum inflammatory cytokines and protein expressions (SIRT1, acetylated p65, phosphorylated p65, tissue factor) were measured.
• Effects of resveratrol (SIRT1 agonist) and EX527 (SIRT1 inhibitor) were assessed.

Main Results:

• IVC stenosis led to increased thrombus weight, leukocyte infiltration, and inflammatory cytokines.
• SIRT1 expression decreased, while p-p65, Ace-p65, and TF expression increased post-stenosis.
• Resveratrol reduced thrombus weight and inflammation markers, and increased SIRT1 expression.
• EX527 reversed the protective effects of resveratrol.

Conclusions:

• SIRT1 activation mitigates IVC stenosis-induced DVT through anti-inflammatory mechanisms in rats.
• SIRT1 represents a potential therapeutic target for DVT treatment.