Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Bonds02:43

Peptide Bonds

82.9K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
82.9K
Subcellular Fractionation01:32

Subcellular Fractionation

8.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.9K
Stereoisomerism of Cyclic Compounds02:33

Stereoisomerism of Cyclic Compounds

11.1K
In this lesson, we delve into the role of ring conformation and its stability, which determines the spatial arrangement and, consequently, the molecular symmetry and stereoisomerism of cyclic compounds. 1,2-Dimethylcyclohexane is used as a case study to evaluate the possible number of stereoisomers. Here, given the multiple (n = 2) chiral centers, there are 2n = 4 possible configurations that lack a plane of symmetry, as the ring skeleton exists in a non-planar chair conformation. In addition,...
11.1K
Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

1.6K
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
1.6K
Cyclic Processes And Isolated Systems01:19

Cyclic Processes And Isolated Systems

3.5K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state. 
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.5K
Protein and Protein Structure02:15

Protein and Protein Structure

87.5K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
87.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A cell-permeable nanobody to restore F508del cystic fibrosis transmembrane conductance regulator activity.

Nature chemical biology·2026
Same author

Polyfluoroalkyl-Tagged Cell-Penetrating Peptide-Additives Enhance Intracellular Protein Delivery via Sustained Monomeric Lipid Interaction.

Angewandte Chemie (International ed. in English)·2026
Same author

Modular Vinyl Phosphonamidates for Cysteine-Directed Protein Targeting.

Journal of the American Chemical Society·2026
Same author

Unsaturated Phosphorus Electrophiles to Probe Protein Tyrosine Phosphatases.

Angewandte Chemie (International ed. in English)·2026
Same author

Expanding the payload scope in antibody-drug conjugates by delivery of hydroxy-containing drugs through self-immolative phosphoramidates.

Nature communications·2026
Same author

High-Affinity Peptide-Drug Conjugate Ligands for the TRIM24 PHD and Bromodomain.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025

Related Experiment Video

Updated: Jan 31, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.8K

Targeted Subcellular Protein Delivery Using Cleavable Cyclic Cell-Penetrating Peptides.

Anselm F L Schneider1,2, Antoine L D Wallabregue1, Luise Franz1,2

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) , Robert-Rössle-Strasse 10 , 13125 Berlin , Germany.

Bioconjugate Chemistry
|January 9, 2019
PubMed
Summary

Cyclic cell-penetrating peptides (cCPPs) enable direct cytosolic delivery of functional proteins like mCherry. Conjugation via a disulfide bond ensures precise subcellular localization, such as to the nucleus or actin cytoskeleton.

More Related Videos

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.2K

Related Experiment Videos

Last Updated: Jan 31, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.8K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.2K

Area of Science:

  • Cell biology
  • Biotechnology
  • Molecular medicine

Background:

  • Direct delivery of functional proteins into living cells remains a significant challenge.
  • Cyclic cell-penetrating peptides (cCPPs) offer a promising non-endosomal pathway for cytosolic protein delivery.
  • Achieving specific subcellular localization of delivered proteins is crucial for functional studies.

Purpose of the Study:

  • To achieve direct cytosolic delivery of the fluorescent protein mCherry using cCPPs.
  • To demonstrate subcellular targeting of delivered mCherry to specific intracellular compartments.
  • To investigate the impact of conjugation strategy on protein localization.

Main Methods:

  • Conjugation of cCPPs to mCherry for cellular uptake.
  • Genetic fusion of targeting peptides to mCherry for subcellular localization.
  • Utilizing disulfide bond conjugation for controlled intracellular release and localization.

Main Results:

  • Efficient cytosolic delivery of mCherry was achieved using cCPPs.
  • Subcellular targeting of mCherry to the nucleus and actin cytoskeleton was successful.
  • Disulfide bond conjugation was essential for precise intracellular localization of mCherry.

Conclusions:

  • cCPPs are effective vehicles for delivering functional proteins into the cytosol.
  • Targeting peptides can direct delivered proteins to specific subcellular locations.
  • Disulfide bond linkage is critical for achieving accurate protein localization within living cells.