Related Experiment Video
Updated: Jan 31, 2026
07:39
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
Published on: June 4, 2019
8.0K
Tracking of Mitochondrial Endogenous Ribonucleic Acid in the Cancer Cells and Macrophages Using a Novel
Yong Liu1, Jie Niu1, Weishan Wang1
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering , University of Jinan , Jinan , Shandong 250022 , P. R. China.
Analytical Chemistry
|January 9, 2019
Summary
Researchers developed a novel probe, MR-IDE, to track mitochondrial RNA (mtRNA) in cells. This breakthrough enables visualization of mtRNA dynamics in cancer cells and macrophages for the first time.
Area of Science:
- Mitochondrial biology
- Molecular biology
- Biochemistry
Background:
- Mitochondrial RNA (mtRNA) is crucial for gene expression within mitochondria.
- Current methods for tracking mtRNA in living systems are limited, hindering research.
- Understanding mtRNA localization and dynamics is essential for cellular function.
Purpose of the Study:
- To develop a novel, effective method for tracking endogenous mitochondrial RNA (mtRNA) in live cells.
- To enable visualization of mtRNA in specific cell types, including cancer cells and macrophages.
- To overcome the limitations of existing techniques for studying mtRNA.
Main Methods:
- Construction of a low molecular weight probe named MR-IDE.
- Application of the MR-IDE probe for tracking endogenous mtRNA.
- Utilizing the probe in cancer cells and macrophages.
Main Results:
- The MR-IDE probe successfully tracked endogenous mtRNA in biological systems.
- This is the first reported method to visualize mtRNA in cancer cells and macrophages.
- The probe's low molecular weight facilitates efficient cellular uptake and tracking.
Conclusions:
- The MR-IDE probe represents a significant advancement in mtRNA research.
- This new tool allows for unprecedented insights into mtRNA behavior in cellular contexts.
- Future studies can leverage MR-IDE to explore mtRNA's role in disease and cellular processes.
Related Concept Videos
03:05Acid Strength and Molecular Structure
33.1K
Binary Acids and Bases
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
33.1K
02:34Molecular Structure and Acidity
20.8K
An acid can be deprotonated to form a conjugate base or an anion. If the produced anion is more stable, then the acid is stronger. On the contrary, if the anion is unstable, then the acid is weaker. Hence, to determine the acidity of the compound, the stability of its conjugate base is studied using various factors.
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
20.8K
02:43Nucleic Acids
50.2K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
50.2K
02:43Nucleic acids
189.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
189.6K
02:59Animal Mitochondrial Genetics
9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
16.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K