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Updated: Jan 31, 2026

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
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Improved accuracy in DFS pattern interpretation using a novel HEp-2 ELITE system.

Maria Infantino1, O Shovman2,3, B Gilburd2

  • 1Immunology and Allergology Laboratory Unit, S. Giovanni di Dio Hospital, Azienda Usl Toscana Centro, Via Torregalli, 3, 50143, Florence, Italy. maria2.infantino@uslcentro.toscana.it.

Clinical Rheumatology
|January 9, 2019
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Summary
This summary is machine-generated.

A novel HEp-2 substrate improves the accurate interpretation of dense fine speckled 70 (DFS70) and mixed antinuclear antibody (ANA) patterns. This new method enhances DFS70 detection sensitivity and aids in deciphering complex ANA results.

Keywords:
Anti-DFS70Antinuclear antibodiesAutoantibodiesAutoimmune diseaseHEp2-cells

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Area of Science:

  • Immunology
  • Autoimmunity
  • Diagnostic Pathology

Background:

  • Interpreting dense fine speckled 70 (DFS70) and mixed antinuclear antibody (ANA) patterns via conventional HEp-2 immunofluorescence (IIF) presents challenges.
  • A novel HEp-2 IIF substrate (HEp-2 ELITE/DFS70-KO) was developed, combining engineered HEp-2 cells lacking DFS70 autoantigen with conventional HEp-2 cells.

Purpose of the Study:

  • To evaluate the utility and advantages of the novel HEp-2 ELITE/DFS70-KO substrate for ANA screening.
  • To assess its effectiveness in improving the accuracy of DFS70 and mixed ANA pattern interpretation.

Main Methods:

  • One thousand and five routine samples for ANA screening were tested on both standard HEp-2 and HEp-2 ELITE DFS70 KO substrates.
  • Anti-DFS70 antibody specificity was confirmed using immunoblot (IB).
  • Clinical and serological data were analyzed to determine the substrate's impact on DFS pattern interpretation.

Main Results:

  • Of 22 cases with suspected DFS patterns on conventional HEp-2, 17 were interpreted with higher accuracy using the HEp-2 ELITE method.
  • The new substrate correctly identified monospecific DFS70 (10), mixed DFS70 (7), speckled (3), and DFS (2) patterns.

Conclusions:

  • The HEp-2 ELITE/DFS70-KO substrate effectively deciphers unclear mixed ANA patterns.
  • It demonstrates high sensitivity for detecting DFS70 patterns, outperforming conventional methods when compared to IB results.