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Updated: Jan 31, 2026

Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster
Published on: November 21, 2018
Expression and Activity of Calpain A in Drosophila melanogaster
Maira Cardoso1, Danielle Oliveira2, Helena Araujo3,4
1Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Abstract:
Detecting calpain activity in Drosophila tissues is a fundamental tool to study calpain function. We use differential centrifugation to prepare membrane- versus cytosol-enriched fractions for measuring calpain activity with the fluorogenic substrate N-LY-AMC. With this method one can measure calpain A activity in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determined activity measurements. Here we describe the steps for tissue preparation and calpain activity measurement in the Drosophila embryo.
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