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Genetic Models of Calpain Deficiency and Ectopic Expression
Yan Gao1,2, Christine Hall1,2, James MacLeod1,2
1Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
Abstract:
Genetic manipulation in cell and animal models can provide important insights into gene function and the relationships between gene mutation and disease. This chapter describes methods to generate models of calpain-1 and calpain-2 deficiency, or their recombinant ectopic expression in cultured cells, and to genotype a conditional transgenic mouse model of calpain-1/calpain-2 deficiency that can be used to explore physiologic roles for these calpains.
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