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The hemolysin-producer coelomocytes in Holothuria polii
C Canicatti1, D Ciulla, E Farina-Lipari
1Institute of Zoology, University of Palermo, Italy.
Abstract:
Using sodium metrizoate discontinuous gradients, two hemolysin-producer amebocyte populations have been separated from total circulating Holothuria polii coelomocytes. The amebocytes of population 1 are responsible for the production of the calcium-dependent and temperature-labile hemolysin, whereas those of population 2 produce the calcium-independent and temperature-stable one. The intracytoplasmic hemolysins were evidenced also by immunofluorescence. Petaloid and filipodial amebocytes were the only positive cell types.
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