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Cloning and characterization of an AAC(6') gene from Serratia marcescens
H M Champion1, P M Bennett, D A Lewis
1Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol, UK.
The Journal of Antimicrobial Chemotherapy
|November 1, 1988
Abstract:
A gene encoding AAC(6') mediated resistance to netilmicin and amikacin was cloned from the chromosome of a nosocomial strain of Serratia marcescens into the BamHI site of pACYC184. The resistance determinant was localized to a sequence of approximately 1 kb. A 490 base pair BamHI-PvuII restriction fragment from the cloned DNA was used as a probe in Southern hybridization studies. Homology with a 920 base pair fragment of PvuII digested S. marcescens DNA from AAC(6')-producing strains was observed. No homology was detected with aminoglycoside sensitive S. marcescens or Escherichia coli.