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Related Experiment Videos

Lactate dehydrogenase isoenzymes.

M Maekawa1

  • 1Department of Laboratory Medicine, Hamamatsu University, School of Medicine, Shizuoka Prefecture, Japan.

Journal of Chromatography
|July 29, 1988
PubMed
Summary

Lactate dehydrogenase (LD) isoenzyme assays are crucial for diagnosis. Electrophoresis is preferred for its comprehensive resolution, while rapid kinetic and immunochemical methods aid in acute myocardial infarction detection.

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Area of Science:

  • Biochemistry
  • Clinical Diagnostics
  • Analytical Chemistry

Background:

  • Lactate dehydrogenase (LD) isoenzymes are critical biomarkers in clinical diagnostics.
  • Accurate and efficient methods for LD isoenzyme analysis are essential for disease diagnosis.

Purpose of the Study:

  • To review and compare various analytical procedures for LD isoenzyme determination.
  • To highlight the advantages and limitations of different assay methodologies.

Main Methods:

  • Electrophoresis for comprehensive isoenzyme resolution.
  • Chromatography, including High-Performance Liquid Chromatography (HPLC), for speed and accuracy.
  • Immunochemical and kinetic methods for rapid assays, particularly for myocardial infarction.

Main Results:

  • Electrophoresis is favored for its direct observability and resolution of all five LD isoenzymes in a single run.
  • HPLC offers improvements in speed, resolution, precision, and accuracy for chromatographic methods.
  • Kinetic and immunochemical methods are rapid and simple, suitable for specific applications like acute myocardial infarction diagnosis (LD-1 and LD-2).
  • Ion-exchange HPLC presents a promising, faster alternative to electrophoresis.

Conclusions:

  • The choice of LD isoenzyme assay method depends on the clinical context and required diagnostic information.
  • Electrophoretic separation remains the preferred method for general LD isoenzyme analysis.
  • Understanding the strengths and weaknesses of each method is vital for accurate interpretation of diagnostic results.

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