Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
Viral Recombination00:57

Viral Recombination

25.1K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.1K
Viral Structure00:56

Viral Structure

74.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.5K
Second Order systems II01:18

Second Order systems II

406
In an underdamped second-order system, where the damping ratio ζ is between 0 and 1, a unit-step input results in a transfer function that, when transformed using the inverse Laplace method, reveals the output response. The output exhibits a damped sinusoidal oscillation, and the difference between the input and output is termed the error signal. This error signal also demonstrates damped oscillatory behavior. Eventually, as the system reaches a steady state, the error diminishes to zero.
406
First Order Systems01:21

First Order Systems

427
First-order systems, such as RC circuits, are foundational in understanding dynamic systems due to their straightforward input-output relationship. Analyzing their responses to different input functions under zero initial conditions reveals significant insights into system behavior.
When a first-order system is subjected to a unit-step input, its response is characterized by its transfer function. By applying the Laplace transform of the unit-step input to the transfer function, expanding the...
427

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenotypic spectrum and theoretical prime editing analysis of WDR19-mediated retinal degeneration.

Documenta ophthalmologica. Advances in ophthalmology·2025
Same author

SLC37A3-associated retinitis pigmentosa: a case report of clinical features and three-year follow up.

Documenta ophthalmologica. Advances in ophthalmology·2025
Same author

X-Linked CGD Chorioretinitis in Two Young Girls.

Biomedicines·2025
Same author

Ablating VHL in rod photoreceptors modulates RPE glycolysis and improves preclinical model of retinitis pigmentosa.

The Journal of clinical investigation·2025
Same author

Prime Editing Strategy to Install the RPE65 c.1430A>G Dominant Mutation.

Advances in experimental medicine and biology·2025
Same author

Megabase Deletion of the Human EYS Locus Using CRISPR/Cas9.

Advances in experimental medicine and biology·2025
Same journal

Correction: Bulatov et al. Camelpox Virus in Western Kazakhstan: Assessment of the Role of Local Fauna as Reservoirs of Infection. <i>Viruses</i> 2024, <i>16</i>, 1626.

Viruses·2026
Same journal

Correction: Franco et al. Whole Blood Volume-Based Absolute Quantification of HTLV-1 Proviral Load: A Comparative Method Evaluation Study. <i>Viruses</i> 2026, <i>18</i>, 580.

Viruses·2026
Same journal

Correction: Medkour et al. Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission. <i>Viruses</i> 2020, <i>12</i>, 657.

Viruses·2026
Same journal

Burden of Malaria and Dengue Across Global, Asian, and Chinese Populations Based on GBD 2021 Data: A Quantitative Assessment of Importation Risks to China.

Viruses·2026
Same journal

First Report of <i>Orthonairovirus songlingense</i> in <i>Haemaphysalis concinna</i> Ticks from Russia.

Viruses·2026
Same journal

Epidemiological and Virological Characteristics of H9N2 Avian Influenza Virus in Jiangsu Province, China, 2024.

Viruses·2026
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K

Viral Delivery Systems for CRISPR.

Christine L Xu1,2, Merry Z C Ruan3,4, Vinit B Mahajan5,6

  • 1Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, New York, NY 10032, USA. christinexu787@gmail.com.

Viruses
|January 10, 2019
PubMed
Summary
This summary is machine-generated.

Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR)/Cas9 gene editing revolutionizes precision medicine. This review explores viral delivery methods for CRISPR/Cas9, aiding disease research and therapy development.

Keywords:
CRISPRanimal modelsgene editinggene therapyviral vectors

More Related Videos

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
09:00

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

8.6K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Related Experiment Videos

Last Updated: Jan 31, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K
Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
09:00

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

8.6K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Area of Science:

  • Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • CRISPR/Cas9 has emerged as a transformative gene editing technology.
  • Its applications span animal model development, disease mechanism elucidation, and therapeutic target validation.
  • CRISPR/Cas9 is recognized for its potential in genome surgery when paired with viral vectors.

Purpose of the Study:

  • To explore diverse viral mechanisms for delivering CRISPR/Cas9 into cells and tissues.
  • To analyze the advantages and disadvantages associated with each viral delivery method.
  • To review the historical development and recent advancements in CRISPR and viral vector technologies.

Main Methods:

  • Review of scientific literature on CRISPR/Cas9 and viral vector delivery systems.
  • Comparative analysis of different viral vector types (e.g., AAV, lentivirus) for CRISPR/Cas9 delivery.
  • Discussion of gene editing efficiency, immunogenicity, and safety profiles.

Main Results:

  • Various viral vectors offer distinct efficiencies and tropisms for CRISPR/Cas9 delivery.
  • Each viral vector system presents unique benefits and limitations regarding payload capacity and host immune response.
  • Recent developments have improved the safety and efficacy of CRISPR/Cas9 viral delivery.

Conclusions:

  • Viral delivery systems are crucial for translating CRISPR/Cas9 technology into clinical applications.
  • Understanding the nuances of different viral vectors is essential for optimizing gene editing strategies.
  • CRISPR/Cas9, facilitated by viral vectors, holds significant promise for advancing disease research and developing novel therapies.