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Updated: Jan 31, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral
1Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
Impact Statement:
This review summarizes how biomaterial substrate modifications (e.g. chemical modifications like natural coatings, ligands, or functional side groups, and/or physical modifications such as topography or stiffness) can prime the cellular response to nonviral gene delivery (e.g. affecting integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, and intracellular trafficking), to aid in improving gene delivery for applications where a cell-material interface might exist (e.g. tissue engineering scaffolds, medical implants and devices, sensors and diagnostics, wound dressings).
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