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Updated: Jan 31, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Total starch in animal feeds and silages based on the chromatographic determination of glucose
Marcela María Salazar Murillo1, Fabio Granados-Chinchilla2
1Sede de Occidente, Universidad de Costa Rica, Recinto de Grecia, Tacares, 118, 20305, Alajuela, Grecia, Costa Rica.
Abstract:
Starch is an important nutrient in animal feed, and so its analysis is of considerable concern as it is one of the most relevant energy containing fractions. Method AOAC 996.11 was modified to exchange the enzymometric and colorimetric step full approach to a simpler HPLC amine-based column one. The method was optimized and validated for its application in animal feeds and silages. •We demonstrated that the method could be used for quality control for animal feeds and silages•We modified the final incubation time, the initial sample mass, the quantity of enzyme added and buffered, to pH 6.2, the medium to which α-amylase is added.•We applied a chromatographic analysis of the glucose that resulted from starch enzymatic hydrolysis, via a refractive index detector and amine-based chromatographic column.
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