L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation

Methane-triggered lateral root formation is not only a universal event, but also dependent on L-cysteine desulfhydrase-dependent hydrogen sulfide signaling. Whether or how methane (CH₄) triggers lateral root (LR) formation has not been elucidated. In this report, CH₄ induction of lateral rooting and the role of hydrogen sulfide (H₂S) were dissected in tomato and Arabidopsis by using physiological, anatomical, molecular, and genetic approaches. First, we discovered that CH₄ induction of lateral rooting is a universal event. Exogenously applied CH₄ not only triggered tomato lateral rooting, but also increased activities of L-cysteine desulfhydrase (DES; a major synthetic enzyme of H₂S) and induced endogenous H₂S production, and contrasting responses were observed in the presence of hypotaurine (HT; a scavenger of H₂S) or DL-propargylglycine (PAG; an inhibitor of DES) alone. CH₄-triggered lateral rooting were sensitive to the inhibition of endogenous H₂S with HT or PAG. The changes in the transcripts of representative cell cycle regulatory genes, miRNA and its target genes were matched with above phenotypes. In the presence of CH₄, Arabidopsis mutant Atdes1 exhibited defects in lateral rooting, compared with the wild-type. Molecular evidence showed that the transcriptional profiles of representative target genes modulated by CH₄ in wild-type plants were impaired in Atdes1 mutant. Overall, our data demonstrate the main branch of the DES-dependent H₂S signaling cascade in CH₄-triggered LR formation.