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Constitutive binding of yeast heat shock factor to DNA in vivo
1Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
Molecular and Cellular Biology
|November 1, 1988
Abstract:
We measured the binding of yeast heat shock factor (HSF) to DNA in vivo by using an interference assay in which HSF excludes GAL4 from a synthetic promoter element containing overlapping binding sites for each protein. The results show that HSF binds to DNA in unstressed cells and that binding is not sufficient for transcriptional activation.