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Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
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Transcriptome profile with 20 nm silver nanoparticles in yeast.
Cullen Horstmann1, Chelsea Campbell1, Daniel Sungwhi Kim2
1Department of Biology, Missouri State University, 901 S National, Springfield, MO 65807, USA.
FEMS Yeast Research
|January 10, 2019
Summary
Engineered silver nanoparticles (AgNPs) inhibit yeast growth and negatively affect cellular processes. AgNPs disrupt ribosome biogenesis and compromise cell wall and mitochondrial integrity in Saccharomyces cerevisiae.
Area of Science:
- Environmental Science
- Nanotechnology
- Molecular Biology
Background:
- Engineered nanomaterials are common in consumer products, necessitating environmental impact assessments.
- Silver nanoparticles (AgNPs) are widely used, but their biological effects require thorough investigation.
Purpose of the Study:
- To investigate the effects of 20 nm spherical citrate-coated AgNPs on Saccharomyces cerevisiae.
- To understand the molecular mechanisms underlying AgNP toxicity in yeast.
Main Methods:
- Growth assays to determine inhibitory concentrations of AgNPs.
- RNA sequencing (RNAseq) for transcriptome analysis of AgNP-treated yeast.
- Cell wall stability assays and RT-qPCR for gene expression validation.
Main Results:
- AgNPs inhibited yeast growth above 5 μg/mL.
- Transcriptome analysis revealed differential expression of hundreds of genes related to ribosome biogenesis, cell wall, membrane, and mitochondrial functions.
- AgNP exposure led to increased susceptibility to cell wall damage.
Conclusions:
- Sublethal AgNP exposure negatively impacts multiple cellular processes in yeast.
- AgNPs disrupt key cellular functions, including ribosome biogenesis and integrity of cellular structures.
- Understanding these effects is crucial for the responsible use of nanomaterials.
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