Expression and Preparation of a G-Protein-Coupled Cannabinoid Receptor CB2 for NMR Structural Studies

Alexei Yeliseev1

  • 1National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Rockville, Maryland.

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The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
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