Lignosulfonate: A Convenient Fluorescence Resonance Energy Transfer Platform for the Construction of a Ratiometric Fluorescence pH-Sensing Probe
Related Experiment Video
Updated: Jan 31, 2026

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer
Published on: July 25, 2025
Yuyuan Xue1, Zechen Wan, Xinping Ouyang
1College of Chemistry and Chemical Engineering , Taiyuan University of Technology , Taiyuan 030024 , P. R. China.
Abstract:
Lignin is a kind of natural fluorescent polymer material. However, the application based on the fluorescent property of lignin was rarely reported. Herein, a noncovalent lignin-based fluorescence resonance energy transfer (FRET) system was readily constructed by physical blending method with spirolactam rhodamine B (SRhB) and lignosulfonate (LS) as the acceptor and donor groups, respectively. The FRET behavior, self-assembly, and energy transfer mechanism of SRhB/LS composite were systematically studied. It was demonstrated that LS could be used as a convenient aptamer as energy donor to construct water-soluble ratiometric sensors because of its inherent property of intramicelle energy transfer cascades. Our results not only present a facile and general strategy for producing lignin-based functional material but also provide a fundamental understanding about lignin fluorescence to promote the functional and high-valued applications of lignin fluorescence characteristic.
More Related Videos
12:23Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
Related Concept Videos
Energy Transfer in Chemical Reactions
Resonance
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Atomic Fluorescence Spectroscopy
Confocal Fluorescence Microscopy