Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Imaging thrombi with radiolabelled fragment E1.

¹Temple University School of Medicine and Hospital, Philadelphia.

Nuclear Medicine Communications

|October 1, 1988

Summary

Fragment E1, a fibrin fragment, effectively localizes in venous thrombi. This radiolabeled compound shows rapid blood clearance and high thrombus-to-blood ratios, making it a promising diagnostic tool for deep-vein thrombosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hairy polyp of the oropharynx in association with a first branchial arch sinus.

The Journal of laryngology and otology·2012

Same author

Presentation of laryngeal papilloma in childhood: the Leeds experience.

International journal of clinical practice·2012

Same author

The use of ice-lollies for pain relief post-paediatric tonsillectomy. A single-blinded, randomised, controlled trial.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2011

Same author

Endoscopic sinus surgery as day-case procedure.

The Journal of laryngology and otology·2008

Same author

Diode laser for paediatric airway procedures: a useful tool.

International journal of clinical practice·2008

Same author

Gastric emptying of a non-digestible solid: assessment with simultaneous SmartPill pH and pressure capsule, antroduodenal manometry, gastric emptying scintigraphy.

Neurogastroenterology and motility·2008

Area of Science:

Biochemistry
Radiopharmacology
Thrombosis Research

Background:

Fragment E1 is derived from human fibrin, specifically its N-terminal regions.
It possesses unique binding sites that interact with fibrin polymers, not fibrin monomer or fibrinogen.

Purpose of the Study:

To evaluate the efficacy of radioiodine-labeled Fragment E1 as a thrombus-localizing agent.
To assess the diagnostic potential of Fragment E1 in visualizing deep-venous thrombi.

Main Methods:

Controlled plasmin digestion of cross-linked fibrin to produce Fragment E1.
Radioiodination of Fragment E1 (123I-labelled) for in vivo studies.
Animal models (pigs) and human clinical trials for thrombus visualization.

Main Results:

Fragment E1 demonstrated significant localization in venous thrombi in a pig model, achieving high thrombus/blood ratios (up to 100:1).
Human trials showed visualization of deep-venous thrombi within 30 minutes using 123I-labelled Fragment E1.
The compound exhibits rapid blood clearance, enhancing early thrombus detection, and its uptake is unaffected by heparin.

Conclusions:

123I-labelled Fragment E1 is a highly promising radiopharmaceutical for effective thrombus localization.
Its rapid blood disappearance and specific binding contribute to high thrombus-to-blood ratios, aiding early diagnosis.

Related Experiment Videos