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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Double strand DNA functionalized Au@Ag Nps for ultrasensitive detection of 17β-estradiol using surface-enhanced raman
Hongbin Pu1, Xiaohui Xie1, Da-Wen Sun2
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, Guangzhou Higher Education Mega Center, Guangzhou 510006, China.
Abstract:
A detection method for 17β-estradiol (E2) using surface-enhanced Raman scattering (SERS)-based aptamer sensor was presented. Raman reporter molecule Cy3 labeled E2-aptamer and DNA functionalized gold-silver core-shell nanoparticles (Au@Ag CS NPs) offered SERS with high sensitivity and selectivity. Based on the fabricated double strand DNA-immobilized gold-silver core-shell nanoparticles (Au@Ag NPs), SERS signal intensity of Raman reporter changed with the number of Cy3-labeled aptamer attached to the core-shell nanoparticles due to the strong binding affinity between the aptamers and E2 with different concentrations. A wide linear range from 1.0 × 10-13 to 1.0 × 10-9 was obtained for the detection of E2, with a low detection limit of 2.75 fM. This proposed method showed highly sensitive and selective for detecting E2, and could be used to determine E2 in actual samples.
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