Nanoparticle based simple electrochemical biosensor platform for profiling of protein-nucleic acid interactions

Area of Science:

• Biophysics
• Biochemistry
• Biosensor Technology

Background:

• Quantitative analysis of protein-nucleic acid interactions is crucial in biophysics.
• Existing methods for biomolecular interaction analysis often lack simplicity, speed, and accuracy.

Purpose of the Study:

• To establish a novel electrochemical biosensor for quantitative analysis of protein-nucleic acid binding.
• To demonstrate a rapid and accurate method for fabricating protein-based biosensors.

Main Methods:

• Fabrication of nanoparticle-based sensors using inkjet printing and plasma conversion.
• Development of a novel bioconjugation technique for biosensor assembly.
• Electrochemical detection of binding interactions between proteins and nucleic acids.

Main Results:

• The nanoparticle-based electrochemical biosensor accurately quantified the binding interaction between unwinding protein 1 (UP1) and SL3ESS3 RNA.
• The biosensor approach demonstrated accuracy comparable to traditional biophysical methods.
• Distinct binding patterns of G-tract nucleic acid sequences with heterogeneous nuclear ribonucleoprotein H1 were profiled.

Conclusions:

• The developed nanoparticle-based electrochemical biosensor provides a universal platform for in vitro biomolecule interaction analysis.
• This approach offers a simple, rapid, and accurate alternative for studying protein-nucleic acid binding.
• The study validates the utility of inkjet-printed nanoparticle sensors for biophysical research.