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Regional outbreak of community-associated methicillin-resistant Staphylococcus aureus ST834 in Japanese children

Yuki Uehara1,2,3, Takashi Sasaki4,5, Tadashi Baba4,5

  • 1Department of Infection Control Science, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. yuuehara@juntendo.ac.jp.

BMC Infectious Diseases
|January 11, 2019
PubMed
Abstract

Insights

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are a growing concern in Japan. This study identified a specific CA-MRSA strain (ST834) predominantly affecting children, characterized by unique genetic features and antibiotic susceptibility.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) presents a significant global and Japanese health challenge.
  • This study investigated 10 pediatric CA-MRSA cases hospitalized between 2011 and 2014 in Saitama, Japan.

Purpose of the Study:

  • To characterize CA-MRSA strains from hospitalized pediatric patients using whole genome sequencing (WGS).
  • To understand the genetic relatedness and unique features of these CA-MRSA strains.

Main Methods:

  • Isolation and identification of MRSA strains from hospitalized patients.
  • Antimicrobial susceptibility testing, PCR, and PFGE for molecular typing.
  • Whole genome sequencing (WGS) for detailed genetic analysis and phylogenetic reconstruction.

Main Results:

  • Ten CA-MRSA strains were identified from pediatric patients, primarily causing purulent lymphadenitis.
  • All strains belonged to sequence type (ST) 834 or its variants, harbored SCCmec type IVc, and were susceptible to fluoroquinolones and tetracyclines.
  • Phylogenetic analysis revealed close relatedness among strains (35-119 SNPs via WGS). Nine strains possessed a unique genomic island, SaPISaitama2, containing virulence genes.

Conclusions:

  • A regional outbreak of ST834-related CA-MRSA in children was identified in Japan.
  • The unique pathogenicity island (SaPISaitama2) and susceptibility to certain antibiotics may contribute to pediatric tropism.
Keywords:
Community-associated methicillin-resistant Staphylococcus aureusPediatricsPurulent lymphadenitisRegional outbreakSaPISaitama2Sequence type 834

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