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Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
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Two-step stem cell therapy improves bone regeneration compared to concentrated bone marrow therapy.

Julia Bolte1,2, Corina Vater1,2, Anna Carla Culla1,2

  • 1University Center of Orthopaedics and Traumatology, University Medicine Carl Gustav Carus Dresden, Fetscherstraße 74, TU Dresden 01307, Dresden, Germany.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|January 11, 2019
PubMed
Summary

Osteogenically pre-differentiated mesenchymal stromal cells (MSCs) combined with platelet lysate (PL) significantly enhanced bone regeneration and vascularization in mice. This approach outperformed bone marrow concentrate (BMC) for critical-size bone defects.

Keywords:
2-step stem cell therapybone defectconcentrated bone marrow therapyplatelet lysate

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Use of Human Perivascular Stem Cells for Bone Regeneration
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Area of Science:

  • Regenerative Medicine
  • Orthopaedic Research
  • Stem Cell Biology

Background:

  • Adult stem cells show promise for bone regeneration.
  • Current bone marrow concentrate (BMC) therapies yield low cell numbers.
  • Two-step stem cell therapy offers higher progenitor cell yields and osteogenic pre-differentiation.

Purpose of the Study:

  • To investigate the effects of BMC, osteogenic pre-differentiation of mesenchymal stromal cells (MSCs), and platelet lysate (PL) on bone regeneration and vascularization.
  • To compare the efficacy of pre-differentiated MSCs versus BMC for bone defect healing.

Main Methods:

  • Human bone marrow was used to generate BMC or isolate MSCs.
  • Seventy-two mice with femoral bone defects were randomized into six groups (Control, PL, BMC, BMC+PL, pre-differentiated MSCs, pre-differentiated MSCs+PL).
  • Bone regeneration and vascularization were assessed after a 6-week follow-up.

Main Results:

  • The pre-differentiated MSCs + PL group exhibited the highest bone volume, histological healing grade, and bridged defect count.
  • Pre-differentiated MSCs demonstrated superior bone regeneration compared to BMC in critical-size defects.
  • Platelet lysate alone did not significantly influence bone regeneration.

Conclusions:

  • Expanded and osteogenically pre-differentiated MSCs are effective for critical-size bone defect treatment.
  • Pre-differentiated MSCs show greater potential for bone regeneration than BMC.
  • The specific role of PL in enhancing bone regeneration requires further investigation.