[Response of Chromophoric Dissolved Organic Matter Composition to Different Hydrological Scenarios in Large Eutrophic...

Chromophoric dissolved organic matter (CDOM) is a fraction of dissolved organic matter that can strongly absorb light in the ultraviolet and blue regions and plays an important role in the biogeochemical cycling of carbon, nitrogen, and phosphorus. Unraveling the sources, optical composition, and corresponding spatial variabilities of CDOM can improve our understanding of carbon, nitrogen, and phosphorus cycling in lakes and lake water quality management. CDOM spectral absorption and fluorescent excitation-emission matrices were measured to investigate the compositional dynamics of CDOM under different hydrological scenarios. Our results showed that the mean value of dissolved organic carbon (DOC) concentration of (8.11±1.26) mg·L^-1 in the rainy season was significantly higher than that in the dry season (3.53±1.19) mg·L^-1 (t-test, P<0.01), whereas the mean spectral slope S_275-295 of (20.89±1.90) μm^-1 in the dry season was significantly greater than that in the rainy season (19.09±1.81) μm^-1 (t-test, P<0.001). Three fluorescent components were identified using parallel factor analysis, and we further found that the dynamics of the three CDOM components were strongly influenced by hydrological conditions. Fluorescence intensity (F_max) of the terrestrial humic-rich component C2 increased with increasing water levels and rainfall. Significant negative relationships were found between all three fluorescent components and dissolved oxygen (P<0.01), suggesting that all three components served as important substrates for microbial processing. Significant positive relationships were found between the terrestrial humic-rich C2 and tryptophan-like C1 and chlorophyll-a and chemical oxygen demand, indicating that anthropogenic inputs and algal degradation contributed significantly to the CDOM pool in Lake Taihu. We further found a significant positive relationship between DOC concentration and F_max of terrestrial humic-rich C2 (r²=0.58, P<0.001), suggesting that DOC in Lake Taihu was primarily derived from allochthonous input.