Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved assay method for amylase activity using an amylodextrin fraction as substrate.

S Kikumoto

    Carbohydrate Research
    |March 1, 1978
    PubMed
    Summary

    A new method uses amylodextrin to measure amylase activity quickly and simply. This approach offers high reproducibility for human serum amylase assays and a wider linear range than traditional starch methods.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The mechanism of Q-enzyme action and its influence on the structure of amylopectin.

    Archives of biochemistry and biophysics·1979
    Same author

    The TcO4-binding to human serum albumin --the stoichiometry and characteristics of the binding equilibrium.

    Radioisotopes·1977
    Same author

    Paracrystalline glycogen gives a B-type x-ray diffraction pattern.

    Archives of biochemistry and biophysics·1973
    Same author

    [Synthetic culture media for the production of schizophyllan].

    The Japanese journal of antibiotics·1970
    Same author

    Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune.

    Gan·1969

    Area of Science:

    • Biochemistry
    • Enzymology
    • Analytical Chemistry

    Background:

    • Amylase activity assays are crucial for diagnosing pancreatic and salivary gland diseases.
    • Existing methods often require large dilution factors or lack precision.
    • A need exists for a rapid, reproducible, and sensitive amylase assay.

    Purpose of the Study:

    • To develop and validate a rapid and simple method for determining amylase activity.
    • To utilize a specific amylo-oligosaccharide fraction (amylodextrin) as a substrate.
    • To establish a reliable assay for human serum amylase.

    Main Methods:

    • Incubation of sample solution with amylodextrin solution.
    • Estimation of consumed substrate by measuring absorbance difference at 460 nm.

    Related Experiment Videos

  • Comparison of kinetic properties (Km value) with starch substrate.

    • Main Results:

    • The Km value of amylodextrin was approximately half that of starch.
    • The homogeneity of amylodextrin facilitated precise amylase unit specification.
    • The method demonstrated excellent reproducibility for human serum amylase assay.
    • A linear relationship was observed over a wide range of amylase concentrations, minimizing dilution needs.

    Conclusions:

    • Amylodextrin provides a superior substrate for amylase activity determination compared to starch.
    • This novel method is rapid, simple, reproducible, and suitable for clinical diagnostics.
    • The assay's wide linear range and precise unit specification enhance its utility.