Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Repetitive attack by Aspergillus oryzae alpha amylase.

J D Allen, J A Thoma

    Carbohydrate Research
    |March 1, 1978
    PubMed
    Summary

    Aspergillus oryzae alpha-amylase repeatedly breaks down multiple glycosidic bonds in maltodextrins. This study quantifies the enzyme's repetitive hydrolytic action on specific carbohydrate substrates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Bridging research and practice: enhancing regulatory decisions with pragmatic clinical trials in oncology.

    ESMO real world data and digital oncology·2026
    Same author

    Risky decision-making and nonsuicidal self-injury among university students: Examining the role of criticism feedback.

    PloS one·2024
    Same author

    A pre-post trial to examine biological mechanisms of the effects of time-restricted eating on symptoms and quality of life in bipolar disorder.

    BMC psychiatry·2024
    Same author

    Contexts of urgency may go beyond emotion.

    Frontiers in psychiatry·2024
    Same author

    A randomized controlled trial to compare the effects of time-restricted eating versus Mediterranean diet on symptoms and quality of life in bipolar disorder.

    BMC psychiatry·2024
    Same author

    Fine-mapping the immunodominant antibody epitopes on consensus sequence-based HIV-1 envelope trimer vaccine candidates.

    NPJ vaccines·2022

    Area of Science:

    • Biochemistry
    • Enzymology
    • Carbohydrate Chemistry

    Background:

    • Oryzae alpha-amylase is a key enzyme in carbohydrate metabolism.
    • Understanding its substrate specificity and mechanism is crucial for industrial applications.
    • Previous studies have focused on single bond hydrolysis.

    Purpose of the Study:

    • To investigate the hydrolytic action of Aspergillus oryzae alpha-amylase on maltooligosaccharides.
    • To determine if the enzyme can hydrolyze more than one glycosidic bond per encounter.
    • To quantify the extent of repetitive glycosidic bond hydrolysis.

    Main Methods:

    • Utilized uniformly radiolabeled maltotriose to maltodecaose substrates.
    • Studied the action of Aspergillus oryzae alpha-amylase.
    • Quantified the repetitive hydrolytic events through product analysis.

    Main Results:

    • Aspergillus oryzae alpha-amylase demonstrated the ability to hydrolyze multiple glycosidic bonds within a single enzyme-substrate interaction.
    • The extent of this repetitive hydrolysis was successfully quantitated.
    • The enzyme's action is not limited to single glycosidic bond cleavage.

    Conclusions:

    • Aspergillus oryzae alpha-amylase exhibits a repetitive hydrolytic mechanism on maltooligosaccharides.
    • This finding provides new insights into the enzyme's catalytic efficiency and mode of action.
    • The quantitation of repetitive attack is significant for understanding enzyme kinetics and applications.

    Related Experiment Videos